• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    1524726 Pivoted photo-cell mounting; centrifugal analysis; cuvette F HOFFMANNLA ROCHE & CO AG 26 Nov 1975 [29 Nov 1974] 48678/75 Headings G1A and G1B [Also in Division G2] CUVETTE A cuvette 17, Fig. 7, comprises a tubular cell 24 having an end window and a closure 25 which is releasably connected with the other end of the cell. The dimensions of both cell and closure are such that liquids placed therein are retained by capillarity. In use, a reagent is placed in the cell 24 and the closure is fitted. A sample and dilutent are introduced to the closure via an hypodermic needle, but due to said capillarity the reagent and sample do not mix. CENTRIFUGE The cuvettes are inserted into holes in the rim of a rotor 11 (the rotor being removably located at 12 by spring arms 10 to facilitate loading). Upon spinning, the sample is transferred by centrifugal force from closure 25 to cell 24. Plate 26 is raised to fling off closures 25 (dotted), and photo-cell 23 is pivoted downwards to receive light from lamp 21 via successive samples. Water from the thermostatted bath is flung via ports 9a to the rim 16 and returns to the bath via ports 9.
    公开号:SU795521A3
    申请号:SU752193202
    申请日:1975-11-26
    公开日:1981-01-10
    发明作者:Занц Мануель；Ревиллет Жорж
    申请人:Ф.Гоффманн-Ля Рош И Ко Аг (Фирма)；
    IPC主号:
    专利说明:

    . 1 The invention relates to the field of photometry. Cell for photometallic analysis of liquids in a centrifuge, made in the form of receiving chambers in a rotor located at the same angular distance from each other, are known. The closest technical solution to the invention is a cell for photometric analysis of liquids in a centrifuge, comprising a housing with a tubular cavity. for receiving the first component and the bottom, and the cover 2. However, such cells are designed for optical measurements in which the light beam passes through the cell parallel to its axis, therefore the thickness of the layer of the test solution is a constant value, resulting in inevitable errors in the introduction of the total amount of liquid in the cell (for example, one sample and one or several reagents), this causes errors in determining the concentration of a particular substance in a sample, photometrically measured by a known cell. Another disadvantage of the known cuvettes is that after each analysis and before each new load they must be cleaned. The presence of residues in the cuvette has a negative effect on the measurement accuracy. Therefore, the cuvet is necessary at certain times. intervals to completely clean, which requires a lot of time. The aim of the invention is to improve the accuracy and speed up the analysis. This goal is achieved by the fact that in a known cuvette for photometric analysis of liquids in a centrifuge, comprising a housing with a tubular cavity for receiving the primary component and a bottom, and a lid, the tubular mold for receiving the second component is made in the lid, the bottom is made of. transparent material, and the body has at least one hole connecting both cavities. In this case, the bottom is located inside the first tubular cavity. The first and second cavities have the same volume. In addition, the walls of the second cavity are made of a material through which a metal needle can be pierced in a cold state. FIG. Figure 1 shows a cuvette for photometric analysis of the centrifuge capacity, general view; in FIG. 2 -.
    The same, top view; in fig. 3–8 are cuvettes for analysis at different phases of the analysis process.
    The cuvette (see Fig. 1) comprises a rotor body 1 with holes 2 into which cuvettes 3 are inserted. The vertical annular walls 4, 5 of the rotor and the horizontal wall 6 form an annular chamber 7 in which the cuvettes 3 are installed with a capacity of 8 for analysis and a lid 9. The device also contains a light source and a photodetector 10 mounted on the fixed part 1 of the cuvette. The council can be pierced with needles 12, 13 to fill it first and. second components. Seals 14 are provided in the cuvette.
    The device operates as follows.
    The cuvette is composed of two parts. One part is a capacitance.
    8 for analysis of the tubular form, closed at one end by a flat bottom 15 located vertically on the longitudinal axis of the container. This container is made of a transparent material, such as glass, or a transparent synthetic material. It is also possible option in which only the bottom transmits light. In any case, the bottom is preferably offset from the end of the pipe back. Another part, the cuvette is a lid 9 having a tubular cavity, one end of which is closed,
    and the other has an end section with a diameter corresponding to the internal diameter of the open end of the first tubular cavity (chamber 7) of the cuvette. This end section ends with a collar 16, which limits the opening of the cover 9 into the container 8. The lid is made of soft material through which a metal needle, such as an injection needle, can pass through the floor. The cover should not be transparent. The volume of the cavities in the tank 8 and the cover 9 is the same.
    FIG. Figure 3 shows only an analysis tube with a tubular cavity, at the bottom of which a reagent is applied, if it is a liquid (the end of the pipette is shown, which gives the reagent). It is also possible to use solid. Reagents, for example, in a lyophilized form. The cuvettes may also be filled with reagent during their manufacture. In this case, when the reagent is already contained in the cuvette in one form or another, the lid
    9 is inserted into the opening of the container 8 as in a lig. one.
    After that, the rotor 1 is loaded by inserting a cuvette outside and then pushing it towards the rotor axis in the position shown in FIG. 5. Seal 14 encloses tubular container 8 so that
    the annular wall 5 of the annular chamber 7 is sealed.
    The cap 9 is pierced with needles (the ends of the pipettes) 12, 13. Moreover, the needle 12 is the end of the pipette that dispenses the reagent, and the needle 13 is the exit. A needle is used to sample the fluid to be analyzed, then a certain amount of water for dilution. Injecting a sample with a needle has the advantage that the needle tip is cleaned from the outside, and internal cleaning occurs when water is removed for dilution. This water, in addition, provides non-stop flow of the sample into the cuvette 3.
    The inner diameter of the lid 9 is chosen so that, due to the surface tension of the injected fluid, a meniscus is formed to prevent the liquid from entering the container 8. Thus, the entire sample and all the water are in the lid 9. The volume of this container basically corresponds to the volume of the analysis tank 8 .
    Immediately after loading all the samples into the cuvettes, the liquids are overloaded by short centrifugation into a container8, where they come into contact with liquid or lyophilized reagents (Fig. 6). Then the solution is mixed and homogenized. For this, oscillatory motions of the rotor are carried out, which causes inside the cuvette 3 (Fig. 7) a strong disturbance of liquids, or liquids and solid reagents. Because of this disturbance, the liquids are mixed and / or the solid reagent is dissolved in the liquid.
    Thereafter, the rotor rotates at a constant speed. Centrifugation is intended to degass the solution by removing lighter than liquid bubbles. These bubbles cause a disturbance of the fluid. The centrifugation also serves to transfer the entire volume of the solution into the container 8. In this case, the caps 9 are discharged by means of a mechanism (not shown in the drawing). .
    To adjust the temperature of liquids in the cells, the cell has a water circulation system (not shown in drawing E.) During the operation of the device, water (see Fig. 8) penetrates into the chamber b. Centrifugal acts on this water during operation of the device force, in the result of which the water enters the chamber b and washes the cuvette. The light from the light source 17 passes through the optical system 18 and the cuvette and hits the photodetector 10.
    权利要求:
    Claims (2)
    [1]
    In the proposed cuvette, the length of the liquid layer, through which the light beam passes, in the tank 8 is proportional to the volume of the solution. Thus, the measurement accuracy does not depend on the amount of reagents contained in the liquid. Accuracy depends only on the number of sample and the diameter of the cavity in vessel 8, for reloading, the rotor is separated from the device. In this case, for each individual analysis, only the cuvettes are changed. Since they are closed, it is advisable to load them with lyophilized reagents or suitable for analysis, liquids in advance. The cuvette is cheap, so it can be thrown away after use. When fluids are introduced into the cavity of a needle test container, the lid prevents fluid from flowing when the rotor oscillates. The caps are easily removed after the mixing and homogenization process is carried out. The displacement of the bottom back relative to the end of the tubular cavity protects the window (s) from dirt, which reduces the light transmission and, consequently, the measurement accuracy. . Claim 1. A cuvette for photometric analysis of liquids in a centrifuge, co0) C o C Q holding a casing with a tubular cavity for receiving the first component and the bottom, and a lid so that In order to increase the accuracy and speed up the analysis, a tube-shaped cavity is made in the lid to receive the second component, the bottom is made of a transparent material, and the case has at least one hole connecting both cavities. 2. The cuve according to claim 1, characterized in that the bottom is located inside the first tubular cavity. 3. A cuvette pop. E, characterized in that the first and second cavities have the same volume. 4. The cuvette of claim I, wherein the walls are second. the cavities are made of a material through which a metal needle can be pierced in a cold state. Sources of information taken into account in the examination 1. For the FRG I 2257069, cl. 42 3/08, published. 1973.
    [2]
    2.Project of the company OPTON, published. 1970, p. 5, 33 (prototype). 567 I / U / F T
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    同族专利:
    公开号 | 公开日
    DE2552883C2|1987-08-20|
    SE414552B|1980-08-04|
    DK145730C|1983-07-25|
    GB1524726A|1978-09-13|
    SE7513328L|1976-05-31|
    DE2552883A1|1976-08-12|
    CH587486A5|1977-05-13|
    US3999868A|1976-12-28|
    DK145730B|1983-02-07|
    AU8690175A|1977-06-02|
    DD122141A5|1976-09-12|
    IT1049795B|1981-02-10|
    JPS5192669A|1976-08-13|
    NL7512789A|1976-06-01|
    AU507010B2|1980-01-31|
    DK530875A|1976-05-30|
    FR2292972B1|1979-08-10|
    NL179235C|1986-08-01|
    JPS5848053B2|1983-10-26|
    FR2292972A1|1976-06-25|
    CA1050300A|1979-03-13|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    EA002403B1|1997-02-28|2002-04-25|Бурштейн Текнолоджис, Инк.|Laboratory in a disk|NL109202C|1959-11-20|
    US3300051A|1963-09-26|1967-01-24|Internat Equipment Co|Filter tube for use in a centrifuge|
    GB1112715A|1965-07-16|1968-05-08|Lancelot Richard Rowett|Disposable urine specimen tube and cap therefor|
    US3844662A|1971-07-13|1974-10-29|Froreich A Von|Sedimentation instrument for body fluids and method of microscopic examination of the sediment|
    US3882716A|1972-07-17|1975-05-13|Elliott Beiman|Centrifugal apparatus and cell|
    US3953172A|1974-05-10|1976-04-27|Union Carbide Corporation|Method and apparatus for assaying liquid materials|DE2553044C3|1975-11-26|1980-04-17|Compur-Electronic Gmbh, 8000 Muenchen|Centrifuge for separating sample liquids|
    WO1982000360A1|1980-07-24|1982-02-04|Oy Labsystems|Method and equipment for the measurement of properties of aliquid|
    DE3134611A1|1981-09-01|1983-03-10|Boehringer Mannheim Gmbh, 6800 Mannheim|METHOD FOR CARRYING OUT ANALYTICAL PROVISIONS AND MEANS SUITABLE FOR THIS|
    US4567373A|1982-10-20|1986-01-28|Shell Oil Company|Centrifugal analyzer|
    US5935522A|1990-06-04|1999-08-10|University Of Utah Research Foundation|On-line DNA analysis system with rapid thermal cycling|
    US5994056A|1991-05-02|1999-11-30|Roche Molecular Systems, Inc.|Homogeneous methods for nucleic acid amplification and detection|
    US5639428A|1994-07-19|1997-06-17|Becton Dickinson And Company|Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay|
    DE19501105A1|1995-01-17|1996-07-18|Hettich Andreas|Arrangement for controlling the temperature of centrifuge rotors|
    ES2434258T3|1996-06-04|2013-12-16|University Of Utah Research Foundation|Apparatus for carrying out PCR and monitoring the reaction in real time during temperature cycles|
    AT312355T|1998-08-17|2005-12-15|METHOD FOR MONITORING PIPETTING PROCESSES|
    BR112018006532A2|2015-10-02|2018-10-16|Bjarne Christian Nielsen Holding Aps|apparatus and method for monitoring and controlling a centrifuge|
    CN106861931B|2017-03-13|2019-04-05|李明|A kind of clinical laboratory's eccentric centrifugal device|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    CH1585874A|CH587486A5|1974-11-29|1974-11-29|
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